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	Provedor de dados OAK (1) Autor Inoue, Noboru (1) Sakurai, Tatsuya (1) Shin-ichiro, Kawazu (1) Tanaka, Miho (1) 井上, 昇 (1) Palavra-chave Epimastigotes (1) In vitro metacyclogenesis (1) Transfection (1) Transgene expression (1) Trypanosoma congolense (1) Ano 2009 (1) País Japan (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Establishment of an in vitro Transgene Expression System in Epimastigotes of　Trypanosoma congolense OAK Sakurai, Tatsuya; Tanaka, Miho; Shin-ichiro, Kawazu; Inoue, Noboru; 井上, 昇. The epimastigote form (EMF) of Trypanosoma congolense appears in the late tsetse infective stage. Epimastigotes adhere to the tsetse proboscis, proliferate in this region, and differentiate into mammal-infective metacyclic forms (MCFs). This differentiation is called metacyclogenesis and is indispensable for cyclical transmission of the parasite. Although an in vitro culture method reproducing metacyclogenesis was established several decades ago, few genetic tools have been utilized to elucidate the molecular mechanisms underlying T. congolense metacyclogenesis. In this study, we established a transgene expression system in the EMF of T. congolense IL3000; the EMF was successfully cultured and observed to undergo metacyclogenesis in vitro. The newly... Palavras-chave: Trypanosoma congolense; Epimastigotes; In vitro metacyclogenesis; Transfection; Transgene expression. Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2677 Registros recuperados: 1 Primeira ... 1 ... Última

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